European Mast Cell and
Basophil Research Network

Mast cells crosstalk with B cells in the gut and sustainIgA response in the inflamed intestine


B lymphocytes are among the cell types whose effector functions are modulated by mast cells (MCs). The B/MC crosstalk emerged in several pathological settings, notably the colon of inflammatory bowel disease (IBD) patients is a privileged site in which MCs and IgA+ cells physically interact. Herein, by inducing conditional depletion of MCs in red MC and basophil (RMB) mice, we show that MCs control B cell distribution in the gut and IgA serum levels. Moreover, in dextran sulfate sodium (DSS)-treated RMB mice, the presence of MCs is fundamental for the enlargement of the IgA+ population in the bowel and the increase of systemic IgA production. Since both conventional B-2 and peritoneal-derived B cells populate the intestine and communicate with MCs in physiological conditions and during inflammation, we further explored this interplay through the use of co-cultures. We show that MCs finely regulate different aspects of splenic B cell biology while peritoneal B cells are unresponsive to the supporting effects provided by MCs. Interestingly, peritoneal B cells induce a pro-inflammatory skewing in MCs, characterized by increased ST2 and TNF-α expression. Altogether, this study uncovers the versatility of the B/MC liaison and highlights key aspects for the resolution of intestinal inflammation. Keywords: IgA; cell-to-cell interplay; colitis; innate-like B cells; mast cells.


Eur J Immunol. 2021 Feb;51(2):445-458. doi: 10.1002/eji.202048668. Epub 2020 Oct 14. Mast cells crosstalk with B cells in the gut and sustain IgA response in the inflamed intestine Viviana Valeri, Silvia Tonon, Shamila Vibhushan, Alessandro Gulino, Beatrice Belmonte, Monika Adori, Gunilla B Karlsson Hedestam, Gregory Gautier, Claudio Tripodo, Ulrich Blank, Francesca Mion, Carlo E M Pucillo PMID: 32920851 DOI: 10.1002/eji.202048668


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